Day 0 – Thursday, 15 September 2022

17.00 Welcome Reception and participants’ registration
18.30 Welcome Cocktail
20.30 End of the day

Day 1 – Friday, 16 September 2022

08:00 Registration
09:00 Opening Ceremony Nicola D’Alterio
Director General, Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise, Teramo, Italy
Jwan Costantini
Major of Giulianova, Teramo, Italy
Marco Marsilio
President of the Abruzzo Region, Italy
Pierdavide Lecchini
Director General, Animal health and veterinary drugs, CVO, Ministry of health, Rome, Italy
Simona Forcella
Policy Officer, European Commission – Directorate-General For Health And Food Safety – Unit G2 – Animal Health, Brussels, Belgium
Frank Boelaert (vdc)
Senior scientific officer, Biological Hazards & Animal Health and Welfare unit (BIOHAW), European Food Safety Authority (EFSA), Parma , Italy
Keith Sumption
Chief Veterinary Officer/ Leader Animal Health Programme (NSAH) Chief, Joint Zoonotic Diseases and AMR Centre (CJWZ), Food and Agriculture Organization of the United Nations (FAO), Rome. Italy
Romano Marabelli
Advisor to the Director General, World Organization for Animal Health (WOAH), Paris, France
Fabrizio De Massis (conference chair)
WOAH and National Reference Laboratory for Brucellosis Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise, Teramo, Italy
11:00 Coffee Break

Session 1: Taxonomy, Evolution, Emerging Species

Chair: Adrian Whatmore
Animal and Plant Health Agency, Addlestone, Surrey, UK.

11:30 Keynote Lecture:

The ever expanding Brucella genus

David O’Callaghan
VBIC, INSERM U1047, University of Montpellier, National Reference Center for Brucella, Microbiology laboratory, Nimes, France
12:00 The global spread of the most famous Brucella species Jeffrey Foster
Pathogen and Microbiome Institute, Northern Arizona University, Flagstaff, USA
12:15 East African Brucella phylogenomics: introducing OrthoPhylo, A turn‐key tool for generating ortholog based bacterial whole genome phylogenetic trees Earl Middlebrook
Los Alamos National Labs, Los Alamos, US
12:30 Canine Brucellosis in France due to Brucella canis: an emerging disease? Guillaume Girault
EU, WOAH & National Reference Laboratory for Animal Brucellosis, Animal Health Laboratory, Paris-Est University/ANSES, Maisons-Alfort, France
12:45 Evolution towards pathogenicity: phylogenomic insights into Brucellaceae Maite Loperena-Barber
Institute of Tropical Health (ISTUN), University of Navarra, Spain
13:00 Brucellosis on the Guiana Shield: emergence of a new Brucella species? Anne Keriel
University of Montpellier, INSERM / Hospital Center of Nîmes, France
13:15 Atypical Brucella spp. are facultatively anaerobic in the presence of nitrates, correlating with increased expression of denitrification genes under anoxic conditions Stephan Köhler
Infectious Diseases Research Institute
(IRIM), CNRS – University of Montpellier, France
13:30 Lunch break/Poster session (Session 1)

Session 2: Genomics and Proteomics

Chair: Giuliano Garofolo

Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise “G. Caporale”, Teramo, Italy.

15:30 Keynote Lecture:
Next-generation proteomics in the fight against pathogens: a guide tour
Jean Armengaud
National Institute of Agronomic Research (INRA), Bagnols sur Cèze, France
16:00 Brucella Databases at BioCyc.org: Pathways, Omics Tools, and Enhanced Genome Annotation Ron Caspi
Bioinformatics Research Group, SRI
International, Menlo Park, California, USA
16:15 Histidine metabolism and metal homeostasis in Brucella abortus Charline Focant
Research Unit in Microorganisms Biology
(URBM), University of Namur, Belgium
16:30 Metabolic Glycoengineering: A strategy to label Brucella abortus cell envelope with synthetic analogues of D-mannose Angeline Reboul
Research Unit in Microorganisms Biology
(URBM), University of Namur, Belgium
16:45 Targeted methodology for the identification of protein‐peptide vaccine candidates Louisa B. Tabatabai (vdc)
Department of Biochemistry, Biophysics and
Molecular Biology, Iowa State University, Ames
Iowa, USA
17:00 Whole genome based tools in epidemiological surveillance of Brucella abortus, Brucella melitensis and Brucella suis bv2 in Italy in the last decade Anna Janowicz
WOAH and National Reference Laboratory
for Brucellosis, Istituto Zooprofilattico
Sperimentale dell’Abruzzo e del Molise,
Teramo, Italy
17:15 In silico pipeline for protein comparisons in Brucella genus Vitomir Djokic
EU, WOAH & National Reference Laboratory
for Animal Brucellosis, Animal Health
Laboratory, Paris-Est University/ANSES,
Maisons-Alfort, France
17:30 Coffee Break/Poster Session (Session 2)
19:00 End of the first day

Day 2 – Saturday, 17 September 2022

Session 3: Pathogenesis and Host-Pathogen-Interaction

Chair: Marty Roop
Brody School of Medicine, East Carolina University, Greenville, North Carolina, USA.

09:00 Keynote Lecture:
Metabolic interaction between Brucella abortus and its host
Renée Tsolis
University of California, Davis, California, USA
09:30 BNIP3L‐dependent mitophagy induced by Brucella abortus in host cells is required for bacterial egress Jeremy Verbeke
Research Unit in Cell Biology – Namur
Research Institute for Life Sciences (NARILIS),
University of Namur, Belgium
09:45 RNA‐immunoprecipitation/miRNA‐Seq reveals miRNA‐like, small noncoding RNAs of Brucella suis, translocated into the cytoplasm of infected murine macrophages Stephan Köhler
Infectious Diseases Research Institute (IRIM),
CNRS-University of Montpellier, France
10:00 Lipopolysaccharide synthesis and traffic in the envelope of the pathogen Brucella abortus Caroline Servais
Research Unit in Microorganisms Biology
(URBM), University of Namur, Belgium
10:15 A Brucella effector that modulates host retrograde transport to promote intravacuolar replication Jean Celli
Paul G. Allen School for Global Animal
Health, Washington State University, Pullman,
Washington, USA
10:30 Novel H-NS-like Protein MucR Coordinates Virulence Gene Expression During Host- Association in Brucella spp. Through Silencer/ Counter-Silencer Interactions Ian S. Barton
Department of Microbiology and Immunology,
Brody School of Medicine, East Carolina
University, Greenville, North Carolina, USA
10:45 Structural insights into the NyxA/B effector family Laurent Terradot
Laboratory of Molecular Microbiology and
Structural Biochemistry, Centre National de la
Recherche Scientifique, Université de Lyon, France
11:00 Coffee Break  

Session 4: Immunology

Chair: Paolo Pasquali
Istituto Superiore di Sanità, Roma, Italy.

11:30 Keynote Lecture:
Internal affairs: defining how cytosolic receptors sense Brucella and contributes to host defense
Sergio Costa Oliveira
Federal University of Minas Gerais, Belo
Horizonte, Brazil
12:00 Antibody response elicited by Brucella abortus strain RB51 vaccine in young water buffaloes (Bubalus bubalis) using a triple dose and the WOAH vaccination schedule. A long term trial Flavio Sacchini
WOAH and National Reference Laboratory for
Brucellosis, Istituto Zooprofilattico Sperimentale
dell’Abruzzo e del Molise, Teramo, Italy
12:15 Antibody response elicited by Brucella abortus strain RB51 vaccine in young water buffaloes (Bubalus bubalis) using a triple dose and the vaccination schedule authorised in Caserta province, Italy Fabrizio De Massis
WOAH and National Reference Laboratory
for Brucellosis, Istituto Zooprofilattico
Sperimentale dell’Abruzzo e del Molise,
Teramo, Italy
12:30 Use of brucellin skin test to identify water buffaloes (Bubalus bubalis) vaccinated with the live attenuated vaccine B. abortus strain RB51 Flavio Sacchini
WOAH and National Reference Laboratory
for Brucellosis, Istituto Zooprofilattico
Sperimentale dell’Abruzzo e del Molise,
Teramo, Italy
12:45 Effects of age on immune response induced by Brucella abortus s19 or RB51 vaccination in heifers Elaine M. S. Dorneles
Federal University of Lavras, Brazil
13:00 TIRAP exon 5 polymorphisms influence the outcome of B. melitensis infection in goats Carlos Rossetti
National Institute of Agricultural Technology
(INTA), Buenos Aires, Argentina
13:15 PMNs supports Brucella dispersal with reduced immune recognition Elías Barquero-Calvo
Research Program in Tropical Diseases, School of Veterinary Medicine, National University, Heredia, Costa Rica
13:30 Coffee Break/Poster Session (Session 3-4)  
14:30 International Brucellosis Society Business Meeting  

Session 5: Vaccination

Chair: José Maria Blasco
Agrifood Institute of Aragón (IA2) CITA-University of Zaragoza, Zaragoza, Spain

15:30 Keynote Lecture:
The bumpy road to a Brucella vaccine: the near hits and good catches
Angela Arenas-Gamboa
Texas A&M University, College Station, Texas, USA
16:00 Registered Influenza Viral Vector Based Brucella abortus Vaccine for Cattle in Kazakhstan: Age‐Wise Safety and Efficacy Studies Kaissar Tabynov
Kazakh National Agrarian Research University
(KazNARU), Almaty, Kazakhstan
16:15 Improving Rev1 vaccine safety in pregnant ewes Pilar M. Muñoz
Department of Animal Science, Center for Research and Agrifood Technology of Aragón-IA2, Zaragoza, Spain
16:30 Screening for vaccinal candidates in Brucella canis: A genomic based strategy for selection of potential DNA target regions Ana M. Zúñiga‐Pereira
CIET, Microbiology Faculty, University of San José, Costa Rica
16:45 BM Delta‐pgm, a superior vaccine for the control of brucellosis in small ruminants Diego Comerci
Biotechnological Research Institute, National University of San Martin (IIBUNSAM-CONICET), Buenos Aires, Argentina
17:00 Pathogenesis of Brucella ovis in pregnant mice and protection induced by the candidate vaccine strain B. ovis ΔabcBA Renato de Lima Santos
Department of Veterinary Clinic and Surgery, School of Veterinary Medicine, Federal University of Minas Gerais, Belo
Horizonte, Brazil
17:15 Identification by Transposon-sequencing of essential genes for chronic infection by Brucella in mice Emeline Barbieux
Research Unit in Biology of Microorganisms
(URBM), NARILIS, UNamur, Belgium,
Laboratoire de Parasitologie, Université Libre
de Bruxelles and ULB Center for Research in
Immunology (U-CRI), Gosselies, Belgium
17:30 Brucella abortus RB51 Vaccine Strain and Raw Milk Consumption: An Emerging Public Health Risk Rebekah Tiller
NCEZID/DHCPP/BSPB Zoonoses and Select
Agent Laboratory, Atlanta, Georgia, USA
17:45 Coffee Break/Poster Session (Session 5)  
19:00 End of the second day  

Day 3 – Sunday, 18 September 2022

Session 6: Diagnostics

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Chair: Falk Melzer
Friedrich-Loeffler-Institute, Federal Research Institute for Animal Health, Jena, Germany.

09:00 Keynote Lecture:
The diagnosis of brucellosis
John McGiven
Brucellosis Reference Laboratory, Animal and
Plant Health Agency (APHA), Bacteriology
Department, Addlestone, United
Kingdom
09:30 Investigation into efficacy of rLPS based serodiagnostic antigens Laurence Howells
Brucellosis Reference Laboratory, Animal and
Plant Health Agency (APHA), Bacteriology
Department, Addlestone, United
Kingdom
09:45 Cross-reaction comparative evaluation of five enzyme-linked immunosorbent assay and Golden Standard methods for porcine Brucellosis diagnosis Acacia Ferreira Vicente
EU, WOAH & National Reference Laboratory
for Animal Brucellosis, Animal Health
Laboratory, Paris-Est University/ANSES,
Maisons-Alfort, France
10:00 Machine Learning for MALDI‐TOF MS identification of Brucella Teresa Romualdi
WOAH and National Reference Laboratory for
Brucellosis, Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise, Teramo, Italy
10:15 Swab types and storage conditions affect Brucella recovery and DNA detection Luca Freddi
EU, WOAH & National Reference Laboratory
for Animal Brucellosis, Animal Health
Laboratory, Paris-Est University/ANSES,
Maisons-Alfort, France
10:30 Proteomics-based identification of immunodominant Brucella canis proteins as candidates for serodiagnosis and vaccine development Dirk Hofreuter
Department of Biological Safety, German
Federal Institute for Risk Assessment, Berlin,
Germany
10:45 Preliminary study of interferon gamma test used for diagnosis of brucellosis in the water buffalo Lorena Schiavo
National Reference Centre for Hygiene
and Technology of Breeding and Buffalo
Production, Istituto Zooprofilattico
Sperimentale del Mezzogiorno, Salerno, Italy
11:00 Coffee Break  

Session 7: Human Brucellosis

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Chair: Ignacio Moriyón
Emeritus Professor, Institute for Tropical Health and Department of Microbiology, University of Navarra, Pamplona, Spain

11:30 Keynote Lecture:
Challenges in human brucellosis
Nicholas J. Beeching
Liverpool School of Tropical Medicine, Liverpool, UK
12:00 Brucellosis in a farming community in central South Africa: A longitudinal cohort study (2015-2018) Jennifer Rossouw
National Institute for Communicable Diseases,
Johannesburg, South Africa
12:15 First analysis of antimicrobial resistance in Brucella strains isolated in Italy Katiuscia Zilli
WOAH and National Reference Laboratory
for Brucellosis, Istituto Zooprofilattico
Sperimentale dell’Abruzzo e del Molise,
Teramo, Italy
12:30 Whole Genome Sequencing for Tracing Geographical Origin of Imported Cases of Human Brucellosis in Sweden Tara Wahab
Department of Microbiology, Public Health
Agency of Sweden, Solna, Sweden
12:45 Screening of FDA approved Drugs to treat Brucellosis Nammalwar Sriranganathan
Biomedical Sciences and Pathobiology,
Virginia Tech, Blacksburg, Virginia, USA
13:00 Human brucellosis exposure in confirmed cutaneous anthrax cases, Dien Bien, Vietnam with an update on human prevalence regionally Jason Blackburn
Spatial Epidemiology & Ecology Research
Laboratory, Department of Geography,
University of Florida, Gainesville, Florida, USA
13:15 Going chronic: about a complicated case of pediatric brucellosis being the first ‘endemic’ incident of human brucellosis in Belgium (2021-2022) Marcella Mori
National Reference Centre for Brucella spp.,
Sciensano (Brussels), Belgium
13:30 Lunch Break/Poster Session (Session 6-7)  

Session 8: Epidemiology, Control and Eradication

Chair: Paolo Calistri
Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise “G. Caporale”, Teramo, Italy.

15:30 Keynote Lecture:
Control of Brucellosis in Domestic Ruminants. Yes we can!
Bruno Garin-Bastuji
French Agency for Food, Environmental and
Occupational Health & Safety (ANSES),
Maisons-Alfort, France
16:00 T-Racing: a contact tracing tool for supporting epidemiological investigation during livestock disease outbreaks Luca Candeloro
WOAH and National Reference Laboratory
for Brucellosis, Istituto Zooprofilattico
Sperimentale dell’Abruzzo e del Molise,
Teramo, Italy
16:15 Follow up investigations on non-infected dogs adopted from the B. canis Italian outbreak Fabrizio De Massis
WOAH and National Reference Laboratory
for Brucellosis, Istituto Zooprofilattico
Sperimentale dell’Abruzzo e del Molise,
Teramo, Italy
16:30 Risk factors for the persistence and spread of Brucellosis in buffaloes in the province of Caserta (2015‐2020) Maria Ottaiano
Istituto Zooprofilattico Sperimentale del
Mezzogiorno, Portici, Italia
16:45 Outbreak of bovine brucellosis in the Bargy mountain: special feature of B. melitensis infection in cattle Claire Ponsart
EU, WOAH & National Reference Laboratory
for Animal Brucellosis, Animal Health
Laboratory, Paris-Est University/ANSES,
Maisons-Alfort, France
17:00 Human brucellosis: First calculated estimate of global annual incidence Christopher G. Laine
Department of Veterinary Pathobiology,
College of Veterinary Medicine & Biomedical
Sciences, Texas A&M University, College
Station, Texas, USA
17:15 Insights into the seroprevalence of Brucella canis infection in dogs in Portugal Ana Cristina Ferreira
National Institute of Agricultural and Veterinary Research (INIAV), Oeiras, Portugal
17:30 Coffee Break/Poster Session (Session 8)  
19:30 Gala Dinner
Lectio Magistralis: “Brucellosis and OneHealth: inherited and future challenges”
Ignacio Moriyón
Professor, Institute for Tropical Health and Department of Microbiology, University of Navarra, Pamplona, Spain

Day 4 – Monday, 19 September 2022

Session 9: Wildlife brucellosis

Chair: Jacques Godfroid
The Arctic University of Norway, Tromsø, Norway.

09:00 Keynote Lecture:
Brucellosis in wildlife and livestock
Henriette van Heerden
University of Pretoria, Onderstepoort, South Africa
09:30 Isolation of Brucella suis biovar 2 in a male roe deer (Capreolus capreolus), in central Italy Marco Gobbi
Istituto Zooprofilattico Sperimentale
dell’Umbria e delle Marche, Perugia, Italy
09:45 Brucella ceti infection in cetaceans from Italian Seas: associated lesions and epidemiological data Carla Grattarola
C.Re.Di.Ma., Istituto Zooprofilattico
Sperimentale del Piemonte, Liguria e Valle
d’Aosta, Torino, Italy
10:00 Brucellosis in vampire bats of Costa Rica: Brucella nosferati sp. nov Gabriela Hernandez-Mora
Veterinary Medical Microbiology Unit, National Animal Health Service (SENASA),
Ministry of Agriculture and Livestock, Heredia,
Costa Rica
10:15 Pathogenesis of Brucella suis biovar 1 in the armadillo (Chaetophractus villosus) Luis Ernesto Samartino
University of Salvador, Pilar, Buenos Aires,
Catholic University of Cuyo, San Luis,
Argentina
10:30 Coffee Break/Poster Session (Session 9)  

Closing Ceremony

Chair: Nicola D’Alterio
Director General, IZS Teramo.

11:00 Poster Award from the International Brucellosis Society
12:00 Closing Remarks and Farewell – Closure of the Conference

Download the complete programme in pdf

Programme